de Olivier Thoumine<\/p>\n
<\/p>\n
Afin de cartographier la dynamique et l’organisation \u00e0 l’\u00e9chelle nanom\u00e9trique des complexes d\u2019adh\u00e9rence synaptique, nous avons d\u00e9velopp\u00e9 une strat\u00e9gie polyvalente bas\u00e9e sur des monom\u00e8res de streptavidine (~3 nm) conjugu\u00e9s \u00e0 des fluorophores organiques robustes. Ces nanosondes ciblent des prot\u00e9ines membranaires s\u00e9lectivement biotinyl\u00e9es sur une petite \u00e9tiquette positionn\u00e9e au niveau de leur domaine extracellulaire. L\u2019approche est tr\u00e8s sp\u00e9cifique, et \u00a0permet un \u00a0marquage efficace dans des environnements cellulaires confin\u00e9s avec un encombrement st\u00e9rique r\u00e9duit et aucun biais de r\u00e9ticulation par rapport \u00e0 des sondes multivalentes. Cette strat\u00e9gie est compatible avec la plupart des techniques de super-r\u00e9solution (STORM, STED et PAINT), et en mode multi-spectral.<\/p>\n
L\u2019article a b\u00e9n\u00e9fici\u00e9 d\u2019un research highlight dans Nature Methods et s\u2019accompagne d\u2019un protocole \u00e0 para\u00eetre prochainement dans Nature Protocols (Chamma et al., sous presse). Nous esp\u00e9rons que cette technique pourra \u00eatre adopt\u00e9e par un grand nombre de neuroscientifiques, pour la d\u00e9tection et caract\u00e9risation de structures sub-cellulaires neuronales.<\/p>\n
<\/p>\n
CHAMMA I, LETELLIER M, Butler, Tessier B, Lim KH, Gauthereau I, Choquet D, Sibarita JB, Park S, Sainlos M*, THOUMINE O* (*Co-last authors).<\/p>\n
Mapping the dynamics and nanoscale organization of synaptic adhesion proteins using monomeric streptavidin.<\/p>\n
Nat Comm 2016, Mar 16;7:10773. doi: 10.1038\/ncomms10773.<\/p>\n","protected":false},"excerpt":{"rendered":"
de Olivier Thoumine Afin de cartographier la dynamique et l’organisation \u00e0 l’\u00e9chelle nanom\u00e9trique des complexes d\u2019adh\u00e9rence synaptique, nous avons d\u00e9velopp\u00e9 une strat\u00e9gie polyvalente bas\u00e9e sur des monom\u00e8res de streptavidine (~3 nm) conjugu\u00e9s \u00e0 des fluorophores organiques robustes. Ces nanosondes ciblent des prot\u00e9ines membranaires s\u00e9lectivement biotinyl\u00e9es sur une petite \u00e9tiquette positionn\u00e9e au niveau de leur […]<\/p>\n","protected":false},"author":4,"featured_media":6497,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[25],"tags":[31],"class_list":["post-6500","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-uncategorized","tag-actualite-en"],"publishpress_future_action":{"enabled":false,"date":"2026-04-22 09:02:26","action":"change-status","newStatus":"draft","terms":[],"taxonomy":"category"},"publishpress_future_workflow_manual_trigger":{"enabledWorkflows":[]},"_links":{"self":[{"href":"https:\/\/www.neurosciences.asso.fr\/en\/wp-json\/wp\/v2\/posts\/6500","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.neurosciences.asso.fr\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.neurosciences.asso.fr\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.neurosciences.asso.fr\/en\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/www.neurosciences.asso.fr\/en\/wp-json\/wp\/v2\/comments?post=6500"}],"version-history":[{"count":1,"href":"https:\/\/www.neurosciences.asso.fr\/en\/wp-json\/wp\/v2\/posts\/6500\/revisions"}],"predecessor-version":[{"id":6501,"href":"https:\/\/www.neurosciences.asso.fr\/en\/wp-json\/wp\/v2\/posts\/6500\/revisions\/6501"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.neurosciences.asso.fr\/en\/wp-json\/wp\/v2\/media\/6497"}],"wp:attachment":[{"href":"https:\/\/www.neurosciences.asso.fr\/en\/wp-json\/wp\/v2\/media?parent=6500"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.neurosciences.asso.fr\/en\/wp-json\/wp\/v2\/categories?post=6500"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.neurosciences.asso.fr\/en\/wp-json\/wp\/v2\/tags?post=6500"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}